doc. Ing. Mgr.

Štěpánka Kučková

Ph.D.

docent

Office hours and theses topics

Brief biography

Publications

Tuesdays 13:30 to 14:30 or by an e-mail arrangement

R 206E

About me

I like to travel and if it’s a relaxing vacation, I like places with the possibility of snorkeling. At home, I enjoy working with students who are excited about new discoveries, my cats, and I like to get my hands dirty growing almost anything green. Slogans for life: if you’re going to do something, do it properly 🙂

Education

  • 2015 – Associate professor (biochemistry): Department of Biochemistry and Microbiology, FPBT, VŠCHT in Prague
  • 2006 – Ph.D. (analytical chemistry): Department of Analytical Chemistry, Faculty of Science, Charles University  dissertation “Analysis of pigments and binders in colored layers of works of art”
    2006 – Engineer (biochemistry and biotechnology): Institute of Biochemistry and Microbiology, FPBT, VŠCHT in Prague; diploma thesis “Identification of protein binders in the color layer of works of art by the method of mass spectrometry”
    2003 – Master’s degree (analytical chemistry): Department of Analytical Chemistry, PřF UK Prague; diploma thesis “Analysis of organic pigments and dyes”

Expertise

  • Analysis of protein binders in works of art and historical mortars by mass spectrometry and identification of protein biomarkers of various diseases in plasma

International experience

  • Institut Royal du Patrimoine Artistique, Belgium 2015
  • Dörner Institute, Germany 2014
  • CICS – Fachhochschule Köln – Institut für Restaurierung und Konservierung von Kunst- und Kulturgut, Germany, 2011
  • Universidad Politecnica de Valencia, Spain, 2005

Awards

  • 2015 Marie Curie Fellowship
  • 2014 Humboldt Fellowship
  • 2011 DAAD Fellowship
  • 2005 3rd place, Student Science Conference, VŠCHT Prague;
  • 2004 2nd place, Student Science Conference, VŠCHT Prague;
  • 2003 2nd place, Youth Competition, AV SR Bratislava;
  • 2003 3rd place, Youth Competition, ÚACH AV CR Reg

H-index: 18

1.
Kuckova, S., Cejnar, P., Santrucek, J., & Hynek, R. (2019).

Characterization of proteins in cultural heritage using MALDI–TOF and LC–MS/MS mass spectrometric techniques. Physical Sciences Reviews, 4(5), 20180011.

2.
Kuckova, S., Hynek, R., & Kodicek, M. (2009).

MALDI-MS applied to the analysis of protein paint binders. Organic mass spectrometry in art and archaeology, 165-187.

3.
Kralova, K., Vrtelka, O., Fouskova, M., Smirnova, T. A., Michalkova, L., Hribek, P., ... & Setnicka, V. (2024).

Comprehensive spectroscopic, metabolomic, and proteomic liquid biopsy in the diagnostics of hepatocellular carcinoma. Talanta, 270, 125527.

4.
Kuckova, S., Sandu, I. C. A., Crhova, M., Hynek, R., Fogas, I., & Schafer, S. (2013).

Protein identification and localization using mass spectrometry and staining tests in cross-sections of polychrome samples. Journal of Cultural Heritage, 14(1), 31-37.

5.
Krizova, I., Schultz, J., Nemec, I., Cabala, R., Hynek, R., & Kuckova, S. (2018).

Comparison of analytical tools appropriate for identification of proteinaceous additives in historical mortars. Analytical and bioanalytical chemistry, 410, 189-200.

6.
Kuckova, S., Nemec, I., Hynek, R., Hradilova, J., & Grygar, T. (2005).

Analysis of organic colouring and binding components in colour layer of art works. Analytical and bioanalytical chemistry, 382, 275-282.

7.
Kuckova, S., Crhova, M., Vankova, L., Hnizda, A., Hynek, R., & Kodicek, M. (2009).

Towards proteomic analysis of milk proteins in historical building materials.International Journal of Mass Spectrometry, 284(1-3), 42-46.

8.
Kuckova, S., Sandu, I. C. A., Crhova, M., Hynek, R., Fogas, I., Muralha, V. S., & Sandu, A. V. (2013)

Complementary cross-section based protocol of investigation of polychrome samples of a 16th century Moravian Sculpture by optical, vibrational and mass spectrometric techniques. Microchemical Journal, 110, 538-544.

9.
Hynek, R., Kuckova, S., Hradilova, J., & Kodicek, M. (2004).

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings. Rapid Communications in mass spectrometry, 18(17), 1896-1900.

10.
Krizkova, M. C., Kuckova, S. H., Santrucek, J., & Hynek, R. (2014).

Peptide mass mapping as an effective tool for historical mortar analysis. Construction and Building Materials, 50, 219-225.

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